Simplified method for derivatization of extractable glyphosate and aminomethylphosphonic acid and their determination by high performance liquid chromatography

Authors : Jamilu GARBA, Abd Wahid SAMSURİ, Radziah OTHMAN, Muhammad Saiful AHMAD-HAMDANİ

Pages : 19-30

View : 14 | Download : 7

Publication Date : 2018-04-01

Article Type : Research Paper

Abstract :This work presents a simple procedure for pre-column derivatization of glyphosate and aminomethylphosphonic acid insert ignore into journalissuearticles values(AMPA); and their determination by high-performance liquid chromatography insert ignore into journalissuearticles values(HPLC);. Derivatization was achieved by mixing a solution of 0.02 M FMOC-Cl, 0.05 M borate buffer and glyphosate or AMPA, then shaken for 1 hour, later washed with diethyl ether and ready for analysis. The quantification was performed by HPLC with fluorescent insert ignore into journalissuearticles values(FLD); or diode array detector insert ignore into journalissuearticles values(DAD);. The result of the HPLC-FLD/DAD showed high linearity insert ignore into journalissuearticles values(R 2 ≥ 0.995); of both compounds over eight point’s concentration range and their high recovery from water compared to soil matrixes.  The relative standard deviation insert ignore into journalissuearticles values(RSD); range from 0.1 to 30 % from the aforementioned matrixes. The limit of detection of HPLC-FLD for glyphosate from water, sandy and clay soil was 0.008 mg L -1 , 0.021 and 0.132 mg kg -1 respectively while that of AMPA was 0.004 mg L -1 , 0.74 and 0.224 mg kg -1 . Meanwhile, the limit of detection of HPLC-DAD for glyphosate from water, sandy and clay soils was 0.024 mg L -1 , 0.731 and 0.122 mg kg -1 respectively while that of AMPA for water sample was 0.076 mg L -1 .  This study was unable to determine lower detection limit for AMPA from soil matrixes by HPLC-DAD thus suggested for more repeated extraction for increasing quantification of the compound.
Keywords : Glyphosate, aminomethylphosphonic acid, Derivatization, Analytical method, Water