The biosynthesis of silver nanoparticles with fungal cytoplasmic fluid obtained from Phanerochaete chrysosporium ME446

Authors : Fatma DENİZ, Mehmet Ali MAZMANCI

Pages : 187-192

Doi:10.35208/ert.788891

View : 10 | Download : 5

Publication Date : 2020-12-31

Article Type : Research Paper

Abstract :Over the last few years, the green synthesis of metal nanoparticles insert ignore into journalissuearticles values(NPs); has become the center of attention of researchers. There are eco-friendly techniques to determine the properties of metal nanoparticles, produced by microorganisms or their cytoplasmic fluids. In the present study, fungal cytoplasmic fluid of white-rot fungi Phanerochaete chrysosporium ME446 was used for the biosynthesis of Ag NP. The pH value of growing media of fungi, AgNO 3 concentration and fungal cytoplasmic fluid of Phanerochaete chrysosporium ME446 insert ignore into journalissuearticles values(PC-FCF); ratio were optimized to determine the most effective conditions. The formation of Ag NPs was monitored by UV visible spectrophotometer at a wavelength of 420 nm. Synthesized Ag NPs were characterized at scanning electron microscope insert ignore into journalissuearticles values(SEM);. Optimum conditions for the pH value, AgNO 3 concentration and PC-FCF ratio were determined as 6.0, 1.50 mM and 100%, respectively. The shape and the sizes of nanoparticles, synthesized at optimum conditions, were confirmed by SEM. The shape was spherical, and the sizes were ranged from 26 to 63 nm.
Keywords : Biosynthesis, Phanerochaete chrysosporium ME446, silver nanoparticles