Can Prolonged Incubation of Negative Blood Cultures Show Fungal Positivity?

Authors : Ali Korhan SIĞ, Diğdem ÖZER YILDIRIM, Başak GÖL SERİN, Mustafa GÜNEY

Pages : 543-547

Doi:10.53424/balikesirsbd.1205875

View : 35 | Download : 34

Publication Date : 2023-09-28

Article Type : Research Paper

Abstract :Objective: Standard duration of one set blood culture insert ignore into journalissuearticles values(BC); is a maximum of 5-7 days. The aim of this study was to evaluate “culture-negative” vials with a prolonged incubation insert ignore into journalissuearticles values(max 30 days); time and to observe any mycological growth. Materials and Methods: Routine BCs obtained from adult patients of Balıkesir Atatürk City Hospital for a year period were included. Render BC System BC128 insert ignore into journalissuearticles values(Render Biotech Co. Ltd., Shenzhen, China); were used. Randomly selected vials were re-incubated for additional three weeks by conventional methods. In case of any growth, identifications were done by PhoenixTM 100 system insert ignore into journalissuearticles values(Becton Dickinson, MA, USA); with cornmeal tween 80 agar insert ignore into journalissuearticles values(RTA Laboratories, Kocaeli, Türkiye);. Antifungal susceptibility testing was applied with CLSI disk diffusion method. Results: A total of 6047 BC sets were obtained and randomly chosen 1040+122 negative sets insert ignore into journalissuearticles values(A and B groups, respectively); were included. In group A, none of them had fungal growth. In B insert ignore into journalissuearticles values(ongoing empiric antifungal ≤48h);, only 2 sets showed significant fungal growth, which were observed in 7±2 days, and all strains were identified as Candida glabrata complex and these patients were on empiric fluconazole insert ignore into journalissuearticles values(200 mg/day);. One isolate was susceptible dose dependent; the other one was resistant for fluconazole. Latter sets of these fungemia patients showed positive signals in routine incubation period. Conclusion: Invasive fungal infections are increasingly encountered and isolation capacity and optimization of BCs are crucial. In this study, it was obviously observed that standard incubation period is satisfactory in order to detect almost all fungemia.
Keywords : invazif fungal enfeksiyonlar, mantarlar, Candida, sepsis