Influence of Temperature and Mobile Phase Composition on Retention Properties of the Macroline Antibiotics Clarithromicin and Roxithromcin  in Reversed-phase Liquid  Chromatography

Authors : A. PAPPA LOUİSİ, P. AGRAFİOTOU, G. ZİSSOPOULOU, P. LİATSİ

Pages : 17-19

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Publication Date : 2002-12-01

Article Type : Research Paper

Abstract :The reversed-phase (RP) chromatographic behaviour of the macrolide antibiotics clarithromycin (Clari) and roxithromycin (Roxi) was extensively studied as a function of mobile phase composition - modified with one, two or three of organic solvents - and column temperature. The results of our study provide a satisfactory thermodynamic explanation for the retention processes of these macrolides in RP-HPLC and give additional information on the selection of optimal separation conditions for the determination of Clari in human plasma using Roxi as an internal standard and vice versa
Keywords : clarithromycin, roxithromycin, HPLC retention