Phycocyanin Extraction From Frozen and Freeze-Dried Biomass of Pseudanabaena sp. by Using Mild Cell Disruption Methods

Authors : Berke KISAOĞLAN, Zeliha DEMİREL, Meltem CONK DALAY

Pages : 333-339

Doi:10.33714/masteb.951265

View : 14 | Download : 5

Publication Date : 2021-12-29

Article Type : Research Paper

Abstract :Phycocyanin is a precious, natural, blue coloured pigment-protein complex that has commercial value and wide application in cosmetics, food, and pharmaceutical industries. In the present study, we performed various cell disruption methods insert ignore into journalissuearticles values(ultrasonication, homogenization, freeze/thaw and CaCl2 extraction); for phycocyanin extraction from different forms of biomass of a thermophilic Pseudanabaena sp. that has a high potential to produce high-quality phycocyanin. Using potassium phosphate buffer and ultrasonic bath method, we achieved the highest phycocyanin yield insert ignore into journalissuearticles values(345 mgPC.g -biomass ); from freeze-dried biomass and we obtained increased yield as the duration of application increases. Phycocyanin yields were calculated as 345 mgPC.g -biomass , 255 mgPC.g -biomass and 220 mgPC.g -biomass for 5, 10 and 15 min, respectively. In this study, cell disruption methods have determined significantly more effective on freeze-dried biomass rather than frozen biomass. Phycocyanin content of freeze-dried biomass was analysed after six months of storage and dramatic decrement was observed in the phycocyanin content of the cells.
Keywords : Cyanobacteria, Extraction, Phycobiliprotein, Phycocyanin, Pseudanabaena sp