Green Synthesis Iron Oxide Nanoparticles (Fe@AV NPs) Induce Developmental Toxicity and Anxiety-Like Behavior in Zebrafish Embryo-Larvae

Authors : Mine KÖKTÜRK, Aybek YİĞİT, Ekrem SULUKAN

Pages : 39-50

Doi:10.33714/masteb.1220668

View : 15 | Download : 5

Publication Date : 2023-03-22

Article Type : Research Paper

Abstract :The synthesis of nanoparticles and the usage areas of these nanoparticles show a rapid increase. In addition to the beneficial use of nanoparticles, their toxic effects cannot be ignored. In our study, iron oxide nanoparticle insert ignore into journalissuearticles values(Fe@AV NPs); insert ignore into journalissuearticles values(mean size: 20.852 nm); was synthesized from Aloe vera plant and the developmental toxicity of zebrafish was investigated. Zebrafish embryo-larvae were treated with different concentrations of Fe@AV NPs insert ignore into journalissuearticles values(1, 10, and 50 mg/L); starting at 4 hours after fertilization and continuing until 96 hours, and different developmental parameters insert ignore into journalissuearticles values(such as survival rate, hatchability rates, malformations, and behavior); were examined. In our study, it was determined that Fe@AV NPs caused developmental toxicity in zebrafish embryos depending on the dose increase. More than 60% died at 96 hours, especially in the highest insert ignore into journalissuearticles values(50 mg/L); application group. It was observed that Fe@AV NPs decreased and delayed the success of exiting the chorion depending on the dose increase, and caused various morphological abnormalities insert ignore into journalissuearticles values(like pericardial edema, tail deformation, and scoliosis); in all application groups except the lowest application group insert ignore into journalissuearticles values(1 mg/L);. While 10 mg/L Fe@AV NPs caused sleep-like behaviors during the daytime by decreasing the daytime motility of the larvae, it caused hyperactivity by increasing their nocturnal motility. The results of thigmotaxis, which is an anxiety parameter, were found to increase anxiety at 10 mg/L Fe@AV NPs exposure.Our findings showed that Fe@AV NPs synthesized from Aloe vera plant have in vivo toxicity and their use at concentrations lower than 1 mg/L can be safe in environmental and medical applications.
Keywords : Zebrafish, Iron oxide nanoparticle, Anxiety, Malformation, Nanotoxicology