Investigation of Taxonomic Relationship and Effect of Seasonal Temperature Changes Based on Protein Profiles of Fishes From Beyşehir, Suğla Lakes and Dam Apa

Authors : Emine ARSLAN, Elif GÜLBAHÇE MUTLU

Pages : 244-251

Doi:10.33714/masteb.1281335

View : 37 | Download : 49

Publication Date : 2023-06-30

Article Type : Research Paper

Abstract :Besides traditional methods based on morphological characters, electrophoretic methods such as SDS-PAGE are preferred by taxonomists to make the right decision in the species identification process. In addition, the effect of environmental factors, such as pH, salinity, heat, and temperature on protein profiles are essential in various studies. In this study, we aimed to determine the degree of relationship in some fish species, such as Squalius lepidus, Cyprinus carpio, Carassius gibelio, Pseudophoxinus anatolicus, Tinca tinca, Alburnus orontis, Scardinius erythrophthalmus, Capoeta capoeta, Vimba vimba, Sander lucioperca living in Beyşehir, Suğla lakes and Apa Dam by SDS-PAGE method, and to examine seasonal differences by evaluating the effect of hot/cold water on protein profiles in fish. Although there were common major protein bands in all fish species studied, the presence of species-specific minor protein bands led to the separation of the species. The same fish species distributed in different lakes and dams were different both in minor bands, and changes in protein profiles were observed consequently on the same fish species synthesizing different proteins in different seasons. The data obtained from this study can contribute to systematic classification studies of fish.
Keywords : Fish, Protein profiles, Relationship degree, SDS PAGE, Seasonal changes