Assessing DNA Barcodes for Identification of Pufferfish Species (Tetraodontidae) in Turkish Marine Waters

Authors : Cemal Turan, Mevlüt Gürlek, Deniz Ergüden, Ali Uyan, Serpil Karan, Servet Ahmet Doğdu

Pages : 55-66

Doi:10.28978/nesciences.369538

View : 25 | Download : 9

Publication Date : 2017-12-19

Article Type : Research Paper

Abstract :In Turkish marine waters, pufferfish belongs to Tetraondontidae family are represented with 8 species, Lagocephalus lagocephalus, L. sceleratus, L. spadiceus, L. suezensis, L. guentheri, Sphoeroides pachygaster, Torquigener flavimaculosus and Tylerius spinosissimus. DNA barcoding can be useful in the assessment of cryptic or morphologically similar species of identification which is widespread in marine environment. DNA barcode identification of the eight puffer species of the Tetraodontidae family in Turkish marine waters were examined by using mtDNA sequencing of the amplified partial mitochondrial cytochrome c oxidase I insert ignore into journalissuearticles values(COI); gene. COI contained 189 variable and 337 conservative nucleotides of which 183 were parsimony informative over 526 bp. Mean genetic diversity all species was found 0.18164. The highest and lowest nucleotide divergence was observed L.spadiceus insert ignore into journalissuearticles values(0.0022); and between L. sceleratus, L. suezensis, L. lagocephalus, L. guentheri and S. pachygaster insert ignore into journalissuearticles values(0.0000); respectively. The number of detected different haplotypes were 10 out of 23 sequences, and haplotype diversity was found to be 1.000. 
Keywords : Pufferfish, catch amount, Aegean Sea, Mediterranean Sea, Turkey