Using Response Surface Methodology for Amperometric Glucose Biosensor Construction

Authors : Gül Özyılmaz, Ali Tuncay Özyılmaz, Seda Ağçam

Pages : 1-15

Doi:10.28978/nesciences.379311

View : 12 | Download : 6

Publication Date : 2018-01-04

Article Type : Research Paper

Abstract :In this study, construction of amperometric glucose biosensor was carried out by immobilizing of glucose oxidase insert ignore into journalissuearticles values(GOD); on platinum electrode with 0.09 cm2 surface area which coated with polypyrrole insert ignore into journalissuearticles values(PPy); by cyclic voltammetry technique. Because measured current values in the presence of glucose would be affected from the electrode preparing and working conditions, experimental parameters should be optimized by response surface methodology insert ignore into journalissuearticles values(RSM);. To this, State Ease Design Expert 8.0.7.1. insert ignore into journalissuearticles values(Serial Number:0021-6578); programe was used. PPy synthesis conditions of pyrrole insert ignore into journalissuearticles values(Py); monomer concentration and scan rate were optimized according to current response in presence of glucose. Optimal Py monomer concentration and scan rate for PPy synthesis were determined as 10 mM and 50 mV/s, respectively. Immobilization parameters such as concentrations of chitosan, GOD and glutaraldehyde insert ignore into journalissuearticles values(GAL); also were optimized by RSM as 1.0 %, 4 mg/ml and 0.0625 %, respectively. The digital photos of electrodes at each stage were obtained. All electrodes well characterized in absence and in the presence of glucose by cyclic voltammetry and impedance techniques and it was observed that electrodes were sensitive to glucose molecule. Finally the effect of working pH and applied potential on the current response was investigated by RSM. The highest current response was obsreved when pH of glucose solution and applied potential were 6.0 and 0.8, respectively.
Keywords : Response surface methodology, Pt electrode, Polypyrrole, amperometric biosensor, glucose oxidase