Determination of the Effect of Epigallocatechin Gallate on Oxidative Stress, Apoptosis and Sperm Quality in Rabbit Semen

Authors : Serkan Ali Akarsu, Recep Hakkı Koca, Aslıhan Çakir Cihangiroğlu, Tutku Can Acisu, Ibrahim Halil Güngör, Mustafa Sönmez, Gaffari Türk, Seyfettin Gür, Ali Doğan Ömür, Harika Eylül Esmer Duruel

Pages : 156-162

Doi:10.56766/ntms.1340936

View : 168 | Download : 203

Publication Date : 2023-10-20

Article Type : Research Paper

Abstract :Objectives;In this study, it was aimed to determine the effect of Epigallocatechin gallate (EGCG) on the cryopreservation of rabbit semen. Material and metod; For this purpose, semen was collected from six male New Zealand rabbits. Collected semen were divided into 5 groups as control, 25 µM, 50 µM, 100 µM and 200 µM. Semen samples were reconstituted and frozen after adding EGCG. Frozen straws were then thawed. Results; After freezing and thawing, total motility, progressive motility, and rate of fast and medium speed sperm were significantly higher in the 50 µM group than in the other groups. Static sperm ratio was found to be lower in the 50 µM group than in the other groups. When the flow cytometry results were examined, there was no statistical difference between the groups in terms of the ratio of dead and live sperm. However, the rate of dead sperm with acrosome damage was found to be the lowest in the 50 µM group. High mitochondrial membrane potential sperm ratio was found higher in 50 µM and 100 µM groups compared to other groups (p<0.05). The apoptosis rate was numerically the lowest in the 25 µM and 50 µM groups. While the rate of ROS was highest in the control group, it was significantly reduced in all EGCG support groups. Conclusion; In conclusion, the addition of EGCG rabbit semen to the cryodium improved semen quality.
Keywords : Antioxidants, cryopreservation, epigallocatechin gallate, rabbit, semen