Experimental cell culture models for investigating neurodegenerative diseases

Authors : Ahmi ÖZ

Pages : 835-851

Doi:10.37212/jcnos.683400

View : 12 | Download : 8

Publication Date : 2019-06-23

Article Type : Research Paper

Abstract :Neurological disorders are an important cause of mortality and constitute 11.84% of total deaths globally according to WHO data 2015. It is estimated to increase up to 12.22% in year 2030. Most common NDs can be account for four main groups such as Alzheimer’s disease insert ignore into journalissuearticles values(AD);, Amyotrophic Lateral Sclerosis insert ignore into journalissuearticles values(ALS);, Huntington’s disease insert ignore into journalissuearticles values(HD); and Parkinson’s disease insert ignore into journalissuearticles values(PD);. Among these diseases, only AD is the seventh common death cause worldwide and until recently the therapeutic approaches are still lack to decrease of prevalence. Hence, developing new strategies to understand molecular targets or break down to cascade of cellular degenerative process in the neurodegeneration should be investigated by future studies. In cell culture studies, many types of tissues and cells can be cultivated to be a minimized model to normal or pathophysiological status of disorders. There are lots of methodology or technique to compose efficient and respective neurodegenerative disease models in cell lines such as COS-7, HC2S2, HEK-293, HeLa, Neuro-2a, NSC-34, PC-12, and SH-SY5Y. We indicated best medium formula to growth of neuronal cells as well as differentiation chemicals and time/dosages. In the review it was aimed to summarize not only give information about cell lines, methodological procedures and molecular mechanisms of the diseases but also represent future perspective and offers to this field of neuroscience research.
Keywords : Alzheimers disease, Huntingtons disease, Parkinsons disease, Amyotrophic lateral sclerosis, Cellular models