Evaluation of the Genetic Fidelity of in vitro Raised Plants of Origanum majorana L. Using Random Amplified Polymorphic DNA

Authors : Burcu ÇETİN

Pages : 237-239

Doi:10.18466/cbayarfbe.406873

View : 17 | Download : 13

Publication Date : 2018-06-30

Article Type : Research Paper

Abstract :Determination of the genetic characters of the plants obtained using plant tissue culture methods is important. In this study, the genetic fidelity of the plants, which are obtained by the micropropagation of the shoot tip explants of the Origanum majorana L. plant of medicinal and economical value in Murashige and Skoog (MS) medium which contains 1.0 mg/L Benzil Amino Purin (BAP), 0.1 mg/L naphthaleneacetic acid (NAA), 3% sucrose and 0.7% agar, has been investigated by using random amplified polymorphic DNA (RAPD-PCR) technique. Monomorphic bands were obtained as a result of all of the RAPD-PCR analyses performed. According to the results obtained, no polymorphism was detected among the micropropagated plants. 
Keywords : Genetic fidelity, Medicinal herb, Micropropagation, Origanum majorana L, RAPD