In silico and in vitro evaluation of oxypeucedanin-induced anticancer activity: Mitotoxicity?

Authors : Ali Ergüç, Hayati Okur, Fuat Karakuş, Gökay Albayrak, Ege Arzuk, Şüra Baykan

Pages : 153-161

Doi:10.30569/adiyamansaglik.1325975

View : 161 | Download : 196

Publication Date : 2023-12-31

Article Type : Research Paper

Abstract :Aim: This study aims to evaluate the alterations in Oxypeucedanin (OXY)-mediated anticancer activity in different media. Second aim is to predict the affinity of OXY to electron transfer chain (ETC) complexes. Materials and Methods: MTT and LDH leakage assays were performed with OXY. Molecular docking studies were also conducted to predict the affinity of OXY to ETC complexes. Results: 250 µM OXY reduced viability in glucose media. ≥50 µM OXY decreased viability in galactose media. ≥50 µM OXY increased membrane disruption in galactose media. Molecular docking studies also showed that OXY might possess the capacity to bind to the inhibition sites of Complex I and IV. Conclusion: Galactose-conditioned media exacerbated the OXY-mediated cytotoxicity. Preliminary results suggested that mitotoxicity might take part in anticancer activity. Furthermore, OXY might cause ETC dysfunctions due to selective inhibition of Complex I and IV.
Keywords : Oksipösedanin, Mitotoksisite, Antikanser aktivite, İn siliko