The protective effects of acetyl L - carnitine which added into Histidine - Tryptophan - Ketoglutarate solution on donor uterus

Authors : İlkay DEMİRCAN, Candan ÖZOĞUL, Seda Nur AKYOL, Mustafa Necmi İLHAN, Mustafa KAVUTÇU, Süreyya BARUN, Mustafa BİLGE, İbrahim Murat HİRFANOĞLU

Pages : 561-568

Doi:10.5798/diclemedj.0921.2015.01.0521

View : 20 | Download : 10

Publication Date : 2016-12-25

Article Type : Research Paper

Abstract :Objective: At the present times uterus transplantation is an alternative therapy for women with untreatable uterine-based infertility factors. Before transplantation, the donor organ must stored in some solutions, but they may not adequate for protection. In this study, we investigated the potential protective effects of acetyl L-carnitine, added into histidine-tryptophanketoglutarate (HTK) solution, on rat uterus. Methods: We divided 24 female Wistar Albino rats into four groups (n=6). Their uterine tissues were stored into four different solutions at different periods at 4oC ; Group 1 HTK, 4 hours; Group 2. HTK + acetyl L- carnitine, 4 hours, Group 3. HTK, 24 hours; Group 4. HTK + acetyl L- carnitine, 24 hours. Then tissues from uterus were used for histological and biochemical examination. Results: In the study the number of TUNEL positive cells in group 4 was lower than group 3. Biochemically evaluated TBARS and NOS levels were highest in group 3 however CAT level was highest in group 2. Conclusion: In conclusion, addition of acetyl L-carnitine to HTK solution reversed the histological alterations after 24 hours cold storage on rat uterus.
Keywords : acetyl L carnitine, uterus, transplantation, infertility