Is anti-IgE therapy effective in preventing magnesium and selenium loss in bones of mice with chronic allergic asthma?

Authors : Serkan GÜRGÜL, Can DEMİREL, Ozlem KESKİN, Fatma Betül ŞEKER, Mehmet Yaşar ÖZKARS, Nurten ERDAL, Coşar UZUN, Yahya NURAL

Pages : 319-326

Doi:10.5798/dicletip.1360644

View : 29 | Download : 43

Publication Date : 2023-09-15

Article Type : Research Paper

Abstract :Objective: Chronic allergic asthma insert ignore into journalissuearticles values(CA); is a respiratory disease that affects millions of people worldwide. While there is evidence linking airway hyperresponsiveness and asthma to factors related to bone metabolism, the impact of asthma on bone health is not well understood. Therefore, to explore whether: insert ignore into journalissuearticles values(i); CA causes meaningful changes in bone magnesium insert ignore into journalissuearticles values(Mg); and selenium insert ignore into journalissuearticles values(Se); levels, and if any, insert ignore into journalissuearticles values(ii); anti-IgE insert ignore into journalissuearticles values(anti-immunoglobulin E); treatment has a protective effect against these changes. Methods: In present study used tibia bones from a previous study on CA in mice. A murine model was used to generate CA. Thirty-two BALB/c male mice were randomly divided into four equal sized groups insert ignore into journalissuearticles values(eight mice/group);: control group insert ignore into journalissuearticles values(intact);, CA insert ignore into journalissuearticles values(treated with saline insert ignore into journalissuearticles values(0.9% NaCl);, CA+L-AIgE insert ignore into journalissuearticles values(100 μg of anti-IgE);, CA+H-AIgE insert ignore into journalissuearticles values(200 μg of anti-IgE);. After immunization, saline was administered by inhalation three times a week. Anti-IgE applications were performed intraperitoneally for a total of 8 weeks in five sessions with 15-day intervals. Bone Mg and Se levels are determined by inductively coupled plasma mass spectrometry insert ignore into journalissuearticles values(ICP-MS);, which is used to determine the elemental composition of various samples. Results: Mg levels of CA and CA+L-AIgE groups were significantly decreased compared to the control insert ignore into journalissuearticles values(P<0.01 for both comparisons);. The mean Mg level of the CA+H-AIgE group was close to the control, and the difference was not significant. In all study groups, Se levels were significantly reduced compared to the control insert ignore into journalissuearticles values(P<0.05 for the CA group, P<0.01 for CA+L-AIgE and CA+H-AIgE groups);. No other significant difference was detected among the groups. Conclusion: Our study provides solid evidence of an association between CA and lower levels of Mg and Se in bones. Current data also showed that anti-IgE therapy can partially and dose-dependently prevent Mg loss induced by CA. These results have significant implications for the treatment and management of bone problems associated with asthma, highlighting the potential for anti-IgE use as a viable treatment for preventing and treating bone mineral metabolism abnormalities in asthma patients.
Keywords : Asthma, Anti IgE, ICP MS, Mineral Loss, Magnesium, Selenium