Genotype and Allele Frequencies of Irritable Bowel Syndrome (IBS)-associated Single Nucleotide Polymorphisms among Malays in Malaysia

Authors : Rasmaizatul Akma ROSDİ, Nurfadhlina MUSA, Zalına ZAHARI, Zahrı KHAIRI, Mulham ALFATAMA, Boon Yin KHOO

Pages : 115-122

Doi:10.18678/dtfd.1247845

View : 75 | Download : 78

Publication Date : 2023-08-30

Article Type : Research Paper

Abstract :Aim: Irritable bowel syndrome insert ignore into journalissuearticles values(IBS); defined by chronic or recurrent abdominal pain or discomfort and changes in bowel habits, is the most common functional gastrointestinal disorder. Studies proved that polymorphisms in the genes were one of the key roles in the underlying IBS. This study aimed to investigate the genotypes and allele frequencies of the IBS-associated single nucleotide polymorphism insert ignore into journalissuearticles values(SNP); from the genes GNB3 insert ignore into journalissuearticles values(rs54443); and SCN5A insert ignore into journalissuearticles values(rs8015124); in unrelated, healthy Malays of Malaysia. Material and Methods: The genomic DNA of 404 subjects was set to nested, multiplex, and allele-specific PCR to determine the aforementioned SNPs. The PCR results were validated through the Sanger sequencing analysis. Results: Malays possessed a slightly higher frequency of wild insert ignore into journalissuearticles values(C); than mutant insert ignore into journalissuearticles values(T); alleles in the rs5443 with 56.3 vs 43.7%. However, the frequencies of the alleles were equivalent in the subset of Malay females insert ignore into journalissuearticles values(C-50%, T-50%);. For rs1805124, only 18.6% of Malays carried the mutant allele G with less than 10 subjects being homozygous mutant GG carriers. Concurrently, the Hardy-Weinberg equilibrium of the SNPs in the study was not deviated. Conclusion: IBS is a common gastrointestinal problem that has significantly reduced the life quality of oneself and become an economic burden to societies. Though the mutant alleles were rather low, the IBS-associated polymorphisms, rs5443 and rs1805124 were noted to be commonly present in the Malays. Further research on the local IBS patients is recommended to affirm the association of rs5443 and rs1805124 polymorphisms and the syndrome.
Keywords : Genetik popülasyon, irritabl bağırsak sendromu, Malayca, rs5443, rs1805124