Effect of Hydroxytyrosol on Prdx6 Expression in Diabetic Rat Liver

Authors : Eda Nur ALMALI, Kayihan KARACOR, Hakan SOYLU

Pages : 141-146

Doi:10.18678/dtfd.1259132

View : 49 | Download : 65

Publication Date : 2023-08-30

Article Type : Research Paper

Abstract :Aim: Oxidative stress caused by hyperglycemia, which is the most important complication of diabetes mellitus, causes liver damage. Hydroxytyrosol is a polyphenolic compound abundant in olive oil that protects the liver against oxidative damage. Peroxiredoxin 6 insert ignore into journalissuearticles values(Prdx6); is an anti-oxidative enzyme known to exist in the liver. The aim of this study was to investigate the effect of hydroxytyrosol on Prdx6 expression in diabetes-induced liver injury. Material and Methods: Male Wistar rats were grouped into four as the control group insert ignore into journalissuearticles values(n=10);, hydroxytyrosol group insert ignore into journalissuearticles values(n=10);, streptozotocin group insert ignore into journalissuearticles values(n=10);, and hydroxytyrosol+streptozotocin group insert ignore into journalissuearticles values(n=10);. Blood glucose levels of the animals were measured after streptozotocin injection and at the end of the experiment. The general structure of the liver was examined with a hematoxylin-eosin stain. Prdx6 protein expression was determined with an immunohistochemical method. Results: In the streptozotocin+hydroxytyrosol group, blood glucose level was found to be lower when compared with the streptozotocin group insert ignore into journalissuearticles values(p<0.001);, and histopathological findings in hepatocytes were found to decrease. Prdx6 expression was found to be similar in the control and hydroxytyrosol groups insert ignore into journalissuearticles values(p=0.590);. However, it was found to be higher in streptozotocin and streptozotocin+hydroxytyrosol groups insert ignore into journalissuearticles values(p<0.001);. Prdx6 expression of the streptozotocin+hydroxytyrosol group was found lower than the streptozotocin group insert ignore into journalissuearticles values(p<0.001);. Conclusion: Hydroxytyrosol, the anti-oxidative activity of which has been proven in many studies, was found to relieve blood glucose levels in diabetic rats, cause histopathological changes in hepatocytes, and decrease anti-oxidative Prdx6 expression. This decrease suggested that instead of inhibiting Prdx6, hydroxytyrosol reduced oxidative stress irrespective of Prdx6.
Keywords : Diyabetes Mellitus, Karaciğer, Hidroksitirozol, Prdx6, Streptozotosin