Screening of Lipid Production Capacities of Bacillus sp. Strains Isolated from Soil and Lipid Staining with Different Staining Techniques

Authors : Elif Demirkan, Irem Yildirim

Pages : 1094-1102

Doi:10.35414/akufemubid.1265060

View : 230 | Download : 191

Publication Date : 2023-10-30

Article Type : Research Paper

Abstract :Microbial lipids have been attracting more and more attention in recent years as promising raw materials for the production of biodiesel and value-added compounds. In the current economic and environmental situation, finding new renewable sources of lipids will be crucial. Recent research has necessitated the search for new efficient microbial producers with lipid production efficiency. For this purpose,50 Bacillus sp. strains previously isolated from the soil were screened for their lipid production capacity. As a result of the production using a single-cell oil production medium, only two Bacillus spp. strains showed growth. These bacteria were named as Bacillus sp. E40 and Bacillus sp. E226. Bacteria were then characterized in terms of their biomass, lipid yields, and lipid contents. The biomass of Bacillus sp. E40 and E226 bacterial isolates, were 0,28 and 0,22 g.L-1, respectively. The highest lipid content was reached by E226 with 54.5%. Bacillus sp. E40 lipid content was determined as 46.4%. In addition, both intracellular lipid staining and colony staining demonstrated the presence of lipids. With this study, it was reported for the first time that local Bacillus sp. strains isolated from soil have lipid producing capacity.
Keywords : Bacillus, Lipid, Boyama, , Sudan Siyahı B