Green Synthesis of Silver Nanoparticles Using Mandragora autumnalis; Its Characterization, Antioxidant and Antimicrobial Activities

Authors : Yeşim DAĞLIOĞLU, Betül ÖZTÜRK

Pages : 1039-1054

Doi:10.18185/erzifbed.915107

View : 20 | Download : 8

Publication Date : 2021-12-18

Article Type : Research Paper

Abstract :The green synthesis approach has benefits over traditional methods in silver nanoparticle synthesis that involve chemical agents interrelated environmental toxicity. For this reason, this approach has been preferred for the biosynthesis of silver nanoparticles (AgNPs). In addition, nanoparticles produced through plants are much more stable, large in size and shape range and can be biosynthesized in high amounts. Silver nanoparticles were synthesized from silver compound (AgNO3) using bioactive compounds of Mandragora autumnalis as capping and reducing agents. Synthesis nanoparticles (Ma-AgNPs) were characterized by SEM, TEM, X-ray diffraction and UV-vis absorption spectroscopy analyzes. X-ray diffraction analysis indicated that the nanoparticles had a crystalline structure. X-ray diffraction analysis demonstrated that the nanoparticles had a crystalline structure. In SEM and TEM images, the spherical shaped and average size of Ma-AgNPs is 20-30 nm. It has been noted that Ma-AgNPs show high antioxidant activity by using DPPH with Ascorbic acid as a standard. It showed a zone of inhibition against Gram positive (Bacillus subtilis, Staphylococcus aureus) and Gram negative (Escherichia coli and Pseudomonas aeruginosa) bacteria.
Keywords : Antioxidant and antimicrobial activity, Disk diffusion, DPPH, Silver nanoparticles, Mandragora autumnalis, Green synthesis