Exploring Genetic Diversity and Population Structure of Turkish Black Sea Region Maize (Zea mays L.) Germplasm using SSR Markers

Authors : Nurettin BARAN, Muhammad Azhar NADEEM, Abdurrahim YILMAZ, Mehtap ANDIRMAN, Fırat KURT, Gültekin TEMİZ, Faheem BALOCH

Pages : 953-963

Doi:10.18185/erzifbed.1128788

View : 15 | Download : 5

Publication Date : 2022-12-30

Article Type : Research Paper

Abstract :Plant landraces are heterogeneous plant populations that have adapted to local environmental conditions and serve as a source of genetic variations that can be used for the breeding perspectives. Studies regarding genetic diversity assessment are very important as they provide an opportunity for the scientific community to investigate novel genetic variations for the genetic improvement of crops. Maize is one of the most important cereal crops and provides daily calories to millions of people all over the world. This study aimed to investigate the genetic diversity and population structure of 32 local maize genotypes collected from the Black Sea Region of Turkey using SSR markers. Molecular characterization was performed using the 14 most polymorphic primers that yielded a total of 42 bands. An average of 3 alleles per SSR primer was detected, and the number of alleles varied from 1 insert ignore into journalissuearticles values(phi022); to 6 insert ignore into journalissuearticles values(umc1571);. The unweighted pair-group method with arithmetic means insert ignore into journalissuearticles values(UPGMA); clustering divided maize accessions into three main populations. According to Nei\`s genetic distances, DZ-M-145 insert ignore into journalissuearticles values(Corum); and DZ-M-20 insert ignore into journalissuearticles values(Trabzon); genotypes were the closest insert ignore into journalissuearticles values(0.03); genetically related populations, while DZ-M-68 insert ignore into journalissuearticles values(Artvin); and DZ-M-55 insert ignore into journalissuearticles values(Rize); were the most genetically distant insert ignore into journalissuearticles values(0.63); populations. The study identified molecular genetic diversity not mentioned for maize plants from the Black Sea. On this occasion, new opportunities have been created for hybrid maize products.
Keywords : SSR, local maize populations, maize, genetic, molecular characterization