IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION

Authors : Gülay YAYCI, Semiha DEDE, Ayşe USTA, Veysel YÜKSEK, Sedat ÇETİN

Pages : 132-137

Doi:10.18036/estubtdc.802794

View : 32 | Download : 13

Publication Date : 2021-07-30

Article Type : Research Paper

Abstract :This study was planned to investigate the effects of glutathione, known to be a strong antioxidant, on caspase-dependent apoptosis and oxidative DNA damage in the kidney cells (BHK-21) exposed to high glucose. BHK-21 cell line was cultivated by regular passages in vitro conditions. Study groups were set up as control, study and its combinations groups ((Glucose; (285 mM, HG), glutathione (250 µM)). After 24 hours of incubation, trypsinized cells were disrupted by freeze-thaw method and analyte was prepared. Caspase 3, 8, 9, M30 and 8-OHdG (oxidative DNA damage marker) levels were determined using commercial ELISA kits. M30, caspase 3, 8 and 9 which are the parameters of apoptosis, were found highest in the HG group. In GSH-treated groups, these parameters decreased slightly. There was no significant difference in the levels of 8-OHdG which is the indicator of oxidative DNA damage.
Keywords : High glucose, glutathione, apoptosis, DNA damage, in vitro, kidney cells