EXOPOLYSACCHARIDE (EPS) ISOLATED FROM ENTEROCOCCUS FAECIUM D36 SHOWS ANTI-CANCER AND ANTI-INVASIVE ACTIVITY POTENTIAL VIA DOWN-REGULATION OF MUC5AC GENE ON HUMAN COLORECTAL ADENOCARCINOMA (CACO-2) CELLS

Authors : Burcugül Altuğ Tasa, Merih Kivanç, Ayşe Tansu Koparal

Pages : 8-17

Doi:10.18036/estubtdc.1265111

View : 42 | Download : 41

Publication Date : 2024-01-30

Article Type : Research Paper

Abstract :To investigate the antiproliferative properties of exopolysaccharides (EPS) on human colorectal adenocarcinoma cell line (Caco-2) and the regulation of MUC5AC gene expression, the antiproliferative effect of EPS isolated from D36 strain was determined by MTT test and the regulation of MUC5AC gene expression was examined using Real-Time PCR. Enterococcus faecium D36 (E. faecium D36) were characterized by Ribotyping analysis. Some biochemical methodologies were preliminarily used to characterize the probiotic potential of E. faecium D36, including morphological, cultural, and physiological characteristics. EPS isolated from E. faecium D36 strain has an antiproliferative effect on Caco-2 cell line, and mucin gene (MUC5AC) expression levels decreased. These results suggest that EPS isolated from E. faecium D36 strain might be a source for a novel anticancer agent. Based on our results, it is believed that EPS obtained from E. faecium D36 can be used as a protective and therapeutic substance during the early stages of cancer, especially colon cancer. EPS affects colon cancer by reducing the invasion ability of cancer by decreasing MUC5AC expression. These findings are thought to shed light on future in vivo studies.
Keywords : Enterococcus faecium, kolorektal kanser, laktik asit bakterileri, Enterococcus faecium, eksopolisakkarit, MUC5AC