Ifosfamide-Loaded Cubosomes: An Approach to Potentiate Cytotoxicity against MDA-MB-231 Breast Cancer Cells

Authors : Popat KUMBHAR, Vıshvajıt KHADE, Varsha KHADAKE, Pradnya MARALE, Arehallı MANJAPPA, Sameer NADAF, Vıjay KUMBAR, Durgacharan BHAGWAT, John DİSOUZA

Pages : 37-52

Doi:10.55262/fabadeczacilik.1145208

View : 16 | Download : 10

Publication Date : 2023-03-01

Article Type : Research Paper

Abstract :Background: Ifosfamide insert ignore into journalissuearticles values(IFS); is proved efficacious against breast cancer, an enormously diagnosed cancer across the globe. However, the clinical efficacy of IFS is limited owing to its hydrophilicity, less stability, and dose-dependent toxicities. Therefore, the primary goal of the present research was to develop IFS-loaded cubosomes with improved anticancer efficacy and reduced dose-dependent toxicities. Methods: The IFS-cubosomes were optimized using a 32 factorial design based on IFS content and zeta potential. The optimized cubosomal dispersion was further assessed for particle size, in vitro IFS release, haemolysis, cytotoxicity, cellular uptake and physical stability. Results: The optimized IFS-cubosomal dispersion exhibited maximum IFS content insert ignore into journalissuearticles values(89.75±4.3%); and better zeta potential value insert ignore into journalissuearticles values(-40.0±1.6 mV);, and size in nanometer. Moreover, IFS-cubosomes retarded IFS release insert ignore into journalissuearticles values(about 91 %); after 12 h than plain IFS solution insert ignore into journalissuearticles values(>99 % within 2 h);. The IFS-cubosomes displayed lower haemolysis insert ignore into journalissuearticles values(3.7±0.79%); towards human RBCs. Besides, the in vitro cytotoxicity of IFS-cubosomes was noticed to be substantially higher insert ignore into journalissuearticles values(IC50: 0.64±0.08 µM); than plain IFS solution insert ignore into journalissuearticles values(IC50: 1.46±0.21 µM); against multi-drug resistant insert ignore into journalissuearticles values(MDR); breast cancer insert ignore into journalissuearticles values(MDA-MB-231); cells. DAPI staining revealed death of IFS-cubosomes treated cells mainly by apoptosis. The cubosomes showed increased uptake by cancer cells. Furthermore, IFS-cubosomes were found to be more stable at refrigeration temperature than at room temperature. Conclusion: Thus, IFS-cubosomes could be a novel avenue in the treatment of breast cancer with improved anticancer efficacy and reduced toxicity. However, further in vivo investigations are desired to validate these claims.
Keywords : Breast cancer, ifosfamide, cubosomes, haemolysis, cytotoxicity, cellular uptake