Parasite-Derived MicroRNAs: Potential Alternative Targets for Laboratory Diagnosis of Cystic Echinococcosis

Authors : Serra ÖRSTEN, İpek BAYSAL, Salih MAÇİN

Pages : 295-302

Doi:10.55262/fabadeczacilik.1324184

View : 33 | Download : 25

Publication Date : 2023-08-01

Article Type : Research Paper

Abstract :Cystic Echinococcosis insert ignore into journalissuearticles values(CE); is a type of zoonotic infection that can be caused by a specific form of a parasite called Echinococcus granulosus sensu lato. Mostly, imaging techniques are utilized to diagnose CE. Serological tests are used only when imaging findings are atypical. Additionally, laboratory assays including direct microscopy and PCR are used in the confirmation of diagnosis after treatment though obtained negative results with these tests cannot be ruled out the diagnosis. Specific miRNAs produced by the parasite could be used as markers to diagnose and monitor CE. This research focuses on investigating the diagnostic potential of parasite-derived miRNAs compared to the presence of protoscolex in animal-derived hydatid cyst samples. Accordingly, egr-let-7-5p, egr-miR71-5p and egr-miR-9-5p were found to be positive in 26, 25 and 11 out of 30 samples insert ignore into journalissuearticles values(86.6%, 83.3% and insert ignore into journalissuearticles values(36.6%);, respectively. There was no relationship between protoscolex presence and detection of either egr-let-7-5p or egr-miR-9-5p insert ignore into journalissuearticles values(p>0.05);. On the other hand, egr-miR71-5p positivity was found statistically significant compared with protoscolex presence insert ignore into journalissuearticles values(p=0.04);. As a result, egr-miR-71 are a promising potential target for the diagnosis of CE. Additional research is necessary to assess the diagnostic value of miRNAs in CE using a larger group of samples.
Keywords : Echinococcus granulosus, kistik ekinokokkoz, tanı, mikroRNA