99mTc-Labeled Microsized Liposomes for Lung Perfusion Imaging

Authors : Helin Hekimoğlu, Merve Karpuz, Emre Özgenç, Evren Gündoğdu

Pages : 491-504

Doi:10.55262/fabadeczacilik.1502918

View : 34 | Download : 66

Publication Date : 2024-10-23

Article Type : Research Paper

Abstract :Respiratory diseases can cause death of the patient if not diagnosed at the early stages. Imaging techniques in nuclear medicine are frequently preferred for the diagnosis of these diseases Radiopharmaceuticals can be used to evaluate the perfusion or ventilation capacity of the lung. Liposomes, formed by lipid layers and an aqueous core, are micro- or nanometer-sized vesicular systems. They can be passively accumulated in lung capillaries due to their surface and physicochemical properties. In the light of this information, in our study, liposome formulations with micrometer particle sizes containing DTPA-PE chelating agent for radiolabeling with 99mTc were designed for the imaging of lung diseases. After the preparation of liposomes, optimum formulation exhibits proper particle size (~5 µm) and zeta potential (~-6 mV) values, and the vesicular integrity was imaged with SEM in characterization studies. Liposomes were dried by lyophilization to obtain cold-kit formulations for radiolabeling, and the effect of the lyophilization process on the characterization properties of the vesicles was evaluated. All liposome formulations were radiolabeled with high efficiency in the optimal radiolabeling conditions comprising 1 mCi of radioactivity, 250 ppm of stannous chloride, and 15 min of incubation period. In addition, radiolabeled liposomes were found to be stable due to their radiolabeling efficiency values which are higher than 90 % for 6 h.
Keywords : Teknesyum 99m, lipozom, akciğer perfüzyon görüntüleme, kapiller blokaj