Cyclophosphamide Exerts an Anti-Metastatic Effect by Reducing the Expression of MMP-2 and -9 in Saos-2 Osteosarcoma Cells

Authors : Gülistan Sanem SARIBAŞ

Pages : 69-74

Doi:10.46332/aemj.1140022

View : 12 | Download : 5

Publication Date : 2023-04-16

Article Type : Research Paper

Abstract :Purpose: Although osteosarcoma is the most common primary malignant bone tumor among adolescents and young adults, the molecular mechanisms underlying the development of cancer are not fully elucidated. Cyclophosphamide insert ignore into journalissuearticles values(CYC); is an alkylating antineoplastic agent widely used in the treatment regimens of various cancers and chronic inflammatory diseases. The aim of this study is to reveal the effect of CYC on Saos-2 cells at the molecular level by determining the expression levels of some matrix metalloprotease proteins insert ignore into journalissuearticles values(MMP-2 and MMP-9); of cancer progression-related genes. Materials and Methods: Cytotoxicity analyzes were performed by applying CYC to the developed human osteosarcoma insert ignore into journalissuearticles values(Saos-2); cell cultures. The determined dose of CYC was applied to the 2D cell lines for 12, 24 and 48 hours. As a result of agent application, the expression levels of MMPs, which are metastatic markers in these cultures, were determined by the immunocytochemical method. Results: While MMP-2 and MMP-9 protein expression levels increased in control insert ignore into journalissuearticles values(PBS); groups in correlation with the extent of duration in the cell cultures created, decreased with CYC administration. Conclusion: It was determined that the expression levels of genes that play a role in metastasis, such as MMP-2 and MMP-9, were decreased by CYC application to Saos-2 cells. These results shed light on molecular studies for the treatment of osteosarcoma cancer.
Keywords : kanser, siklofosfamid, MMP, osteosarkoma, Saos 2