Phytochemical screening and radical scavenging activity of green coffee bean extract: Multiple-protective efficacy against Bisphenol A

Authors : Burhan DURHAN, Emine YALÇIN, Kültigin ÇAVUŞOĞLU, Ali ACAR

Pages : 256-275

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Publication Date : 2021-12-31

Article Type : Research Paper

Abstract :In this study, phytochemical characterization, radical scavenging activity and the protective role of green coffee bean extract (Gcbe) against Bisphenol-A (BpA) were evaluated. In phytochemical analysis total phenolic, flavonoid and tannin contents were investigated and major components were determined by HPLC. The amerolative effect of Gcbe against BpA-induced toxicity were studied in albino mice. For this purpose, mice were separated into six groups which treated with tap-water, BpA and two doses of Gcbe. To determine BpA toxicity and the protective role of Gcbe, changes in some biochemical parameters, micronucleus (MN) frequency and DNA fragmentation were investigated. As a result, the phenolic compounds in the Gcbe content were more intense than the other components. In BpA-treated group, serum parameters increased and the antioxidant/oxidant dynamics were impaired. Gcbe treatment decreased the induced serum parameters and caused an improvement in antioxidant-oxidant dynamics and also a decrease in the DNA-tail percentage and MN frequency.
Keywords : Bisphenol A, Comet, Green coffee bean extract, phytochemical characterization, radical scavenging activity