Investigation of Effect of Rosiglitazone on the Mitochondrial Genome in Rats by Molecular Methods

Authors : Zeliha Cansel ÖZMEN, Muhlise ALVUR, Abdulkerim BEDİR, Birşen BİLGİCİ, Rukiye NAR

Pages : 26-44

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Publication Date : 2023-09-05

Article Type : Research Paper

Abstract :Objectives: Rosiglitazone is a drug which belongs to tiazolidinedione class used in the treatment of Type II diabetes mellitus. The aim of this study was to investigate the effects of rosiglitazone on the mitochondrial DNA by examining the frequency of the mtDNA4834 deletion in the different rat tissues. Material and Methods: Genomic DNA was extracted from liver, small intestine, pancreas tissue and lymphocytes of male Sprague-Dawley rats. Nuclear DNA, mitochondrial DNA and mtDNA4834 deletion copy numbers of the samples was determined by real-time qPCR. mtDNA copy numbers per cell insert ignore into journalissuearticles values(mtDNA/cell); and mtDNA4834 deletion frequency insert ignore into journalissuearticles values(mtDNA4834 deletion frequency insert ignore into journalissuearticles values(%);); were calculation. Results: The mtDNA/cell in the tissues of small intestine and pancreas were significiantly lower than in all rosiglitazone doses compared to the control group insert ignore into journalissuearticles values(p<0.05);. The mtDNA4834 deletion frequency insert ignore into journalissuearticles values(%); in the liver tissue at all doses of rosiglitazone was found to be significiantly lower than in the control group insert ignore into journalissuearticles values(p<0.05);. At the same time, there was a significiant statistical difference in the mtDNA4834 deletion frequency insert ignore into journalissuearticles values(%); and mtDNA/cell between the control groups insert ignore into journalissuearticles values(p<0.05); Conclusion: The results of our study made us think that rosiglitazone has no oxidant effect on this tissue due to the decrease in the frequency of mtDNA4834 deletion in liver tissue. However, the decrease in mitochondrial DNA copy numbers in the small intestine and pancreas tissues may be due to the oxidant effect of rosiglitazone.
Keywords : mtDNA4834 delesyon, mitokondriyal DNA, oksidatif stres, roziglitazon