Rhodobacter sphaeroides O.U.001'den Biyoteknolojik Olarak Üretilen Karotenoidlerin Antioksidan ve Sitotoksik Etkilerinin Araştırılması

Authors : Gökhan KARS, Meltem DEMİREL KARS, İhsan OBALI, Ayça EMSEN, Ufuk GÜNDÜZ

Pages : 559-568

Doi:10.17714/gumusfenbil.597048

View : 23 | Download : 5

Publication Date : 2020-07-15

Article Type : Research Paper

Abstract :Carotenoids take special attention due to their strong antioxidant properties. In the present study, the antioxidant and antiproliferative effects of carotenoids extracted from Rhodobacter sphaeroides O.U. 001 were investigated. For this, R. sphaeroides O.U. 001 was first cultivated in a 5 L bioreactor. After that, the carotenoids were extracted from R. sphaeroides O.U. 001 using acetone as solvent. The effects of carotenoid extract on proliferation of vincristine and paclitaxel resistant cells (MCF-7/Vinc, MCF-7/Pac) were investigated by MTT cytotoxicity assay. The checkerboard plate technique was performed to evaluate the interactions between carotenoid extract and cancer drugs on the cells. The antioxidant activity of the carotenoid extract was assessed by 2, 2 - diphenyl, 1- picryl hydrazyl (DPPH) radical scavenging assay. As a result of extraction, considerable amount of carotenoid yield was obtained (80.69 mg carotenoid/g dry cell weight). It was found that carotenoid extract had no inhibitory effects on cell proliferation and interaction of carotenoids with anticancer drugs exhibited antagonistic effects. In addition to these, the DPPH antioxidant activity (IC 50 value) of the carotenoid extract was calculated as 25 µg/mL. In conclusion, biotechnologically produced bacterial carotenoids were shown to have considerable antioxidant activity without any cytotoxicity on cancer cells.
Keywords : Antioxidant, Bioreactor, Carotenoid, MCF 7, Rhodobacter sphaeroides