Investigation of the inhibition effect of pitaya (hylocereus guatemalensis) bark extracts on some metabolic enzymes

Authors : Rüya SAĞLAMTAŞ

Pages : 595-604

Doi:10.17714/gumusfenbil.1256518

View : 83 | Download : 85

Publication Date : 2023-07-15

Article Type : Research Paper

Abstract :Pitaya is a tropical and subtropical plant with various uses. In this study, the maceration method obtained acetone, ethanol, ethyl acetate, dichloromethane, and methanol from the bark parts of pitaya fruit. The in vitro effects of fruit peel extracts on the critical metabolic enzyme activities of acetylcholinesterase insert ignore into journalissuearticles values(AChE);, butyrylcholinesterase insert ignore into journalissuearticles values(BChE);, human carbonic anhydrase insert ignore into journalissuearticles values(hCA I and hCA II);, and α-glycosidase, were investigated. Pitaya bark ethanol insert ignore into journalissuearticles values(PBE); insert ignore into journalissuearticles values(IC50: 20.16±0.22 µg/mL, r2:0.9694); for AChE and Pitaya bark acetone insert ignore into journalissuearticles values(PBA); insert ignore into journalissuearticles values(IC50:42.10±1.045 µg/mL, r2:0.9807); extract for BChE showed the most potent inhibition effect. Pitaya bark ethyl acetate insert ignore into journalissuearticles values(PBEA); extract showed the most potent inhibitory effect for hCA I and hCA II insert ignore into journalissuearticles values(IC50:53.51±2.22 µg/mL, r2:0.9562 and IC50:26.29±0.34 µg/mL, r2:0.9966);, respectively. In addition, PBEA showed the most potent inhibition effect on α-glycosidase enzyme insert ignore into journalissuearticles values(IC50: 24.54±1.80 µg/mL, r2:0.9199);. According to the results, the extracts can be effectively used treatment of diseases such as glaucoma, epilepsy, type 2 Diabetes mellitus insert ignore into journalissuearticles values(T2DM);, and Alzheimer\`s disease insert ignore into journalissuearticles values(AD);.
Keywords : Enzim, Enzim İnhibisyonu, Pitaya