Phytochemical, in vivo, and in silico Anticonvulsant Activity Screening of Albizia Amara Leave’s Ethanolic Extract

Authors : Ahmed Algali SEDAHMED, Mosab Yahya AL-NOUR, Mirghani Hashim MIRGHANI, Hussam Eldeen ABUALGASIM1, Fadl Albaseer Alamin ALTIB, Ahmed ADİL ALI, Esraa ELHADI, Ahmed Haasan ARBAB

Pages : 9-22

View : 21 | Download : 10

Publication Date : 2021-01-01

Article Type : Research Paper

Abstract :Relying on the previous literature, Albizia amara possesses phytochemical constituents having significant pharmacological activities. Our study preliminary screened the phytochemical constituents and investigated the potential anticonvulsant activity using the Pilocarpine-induced seizures on albino rats. The qualitative phytochemical screening of the extract indicated the presence of flavonoids, alkaloids, saponins, tannins, and sterols. The anticonvulsant activity assessment revealed that the extract (400 mg/kg I.P.) increased the latency to the first seizure and significantly decreased the duration of the seizure. Moreover, the extract reduced the mortality rate during the first observed 24 hours. Furthermore, to predict the probable mechanism of action, in silico study was conducted. The results indicated that predicted compounds that have the highest contribution in the activity are Budmunchiamine A, 1, 2-benzenedicarboxylic acid, mono (2-Ethylhexyl) ester, Hexadecanoic acid methyl ester, and Pithecolobine. The predicted targets that involved in the antiepileptic activity are neural acetylcholine receptor subunits alpha-4 and alpha-7, 5-hydroxytryptamine receptor 3A, Cannabinoid receptor 1, kinase C gamma & epsilon subunits, and Glutamate carboxypeptidase-2 enzyme. Unfortunately, Budmunchiamine A was predicted to be Cardiotoxic and Hepatotoxic. Pithecolobine was predicted to be Cardiotoxic. In conclusion, the findings demonstrate the potentiality of A. amara’s ethanolic leaves extract in the attenuation of Pilocarpine induced convulsion.
Keywords : Albizia amara, phytochemical screening, anticonvulsant activity, Pilocarpine induced seizures, insilico study