New Schiff Base and Its Platinum Complex: Inhibition of Amyloid Aggregation

Authors : Özge Özcan, Salih Günnaz, Sevil İrişli

Pages : 1-11

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Publication Date : 2024-11-25

Article Type : Research Paper

Abstract :This study investigates the synthesis and characterization of a Schiff base (L) comprising amine and imine donor atoms, specifically N1-(4-(benzyloxy)benzylidene)-N2-phenylethane-1,2-diamine, along with its Platinum(II) complex (Pt-L). The structural elucidation of both the ligand and complex is accomplished through various spectroscopic techniques including Fourier-transform infrared spectroscopy (FT-IR), proton nuclear magnetic resonance (1H-NMR), carbon-13 nuclear magnetic resonance (13C-NMR), and elemental analysis. Furthermore, the potential inhibitory effects of the Pt-L complex on Aβ1-42 aggregation are explored using the human neuroblastoma cell line (SH-SY5Y) as a model system. The cytotoxicity of the Pt-L complex on SH-SY5Y neuroblastoma cells is examined, revealing an IC50 value of 19.22 μM. The inhibition kinetics of Aβ aggregation are also investigated fluorometrically using Thioflavine-T. By analyzing the area under the curve, it is calculated that the complex (1.0:1.0 molar ratio) interacts with amyloid at a rate of 65%. These results obtained in our study show that the complex is promising in terms of inhibition of Aβ1-42.
Keywords : Anti Alzheimer aktivitesi, sitotoksisite, schiff bazları, platin schiff bazı kompleksleri