Mutation induction using Co60 in Pembe cekirdeksiz (Vitis vinifera L.) grape cultivar

Authors : Hatice BİLİR EKBİÇ, Semih TANGOLAR, Ercan EKBİÇ

Pages : 101-106

Doi:10.29278/azd.371737

View : 15 | Download : 7

Publication Date : 2017-12-30

Article Type : Research Paper

Abstract :In the study an optimum dose among Co 60 treatments that make less physical injury and positive effects comparing the control plants was investigated in Pembe Cekirdeksiz grape cultivar. Related to this aim, vine cuttings were exposed to the 0, 15, 25, 35 and 45 Gy doses of Co 60 . Buds of radiation treated cuttings were grafted on 17 old vines of Recel grape cultivars (Vitis vinifera L.) by chip bud grafting method. Survival rates obtained from 15 and 25 Gy Co 60 applications were found to be the closest to the control application. 35 and 45 Gy doses of Co 60 were observed as lethal in this cultivar. It was observed that progressive radiation doses resulted in a variation in shoot length and leaf area values. The highest shoot length and leaf area values were obtained from control and 15 Gy Co 60 applications. Radiation treatment made bit late in the bud break but it did not retard the ripening time. On the other hand Co 60 treatment resulted decreasing in cluster and berry size. Stomata numbers decreased with the progressive Co 60 doses. Although same bit variations in different characteristics were determined by radiation treatments, positive variations for grape breeding were not observed at this stage.
Keywords : Grapevine, mutation, gamma irradiation