Evaluation of Antimicrobial Effects of Fresh and Dried Fruit in Turkish Tombul Hazelnut Variety

Authors : Mehtap Usta, Seda Biryol, Ali Soydinç

Pages : 241-248

Doi:10.29278/azd.1355422

View : 134 | Download : 111

Publication Date : 2023-10-29

Article Type : Research Paper

Abstract :Objective: The aim of the study was to determine the antimicrobial properties of fresh and dried fruits of Turkish Tombul hazelnut (Corylus avellana L.) variety. Materials and Methods: The Turkish Tombul hazelnuts used in the experiment were obtained from Helvacı Village/ Düzköy /Trabzon. Fresh hazelnuts were harvested in 2023, while dried hazelnuts were collected in 2022. The dried hazelnuts were dried at 105°C until they attained a consistent weight. This section was not created for fresh hazelnuts. The treated micelle was extracted for 4 hours with 100 mL of Ethyl alcohol (EtOH) for every 10 g of both the fresh and dried hazelnut samples crushed. The micelle (hazelnut+oil+solvent mixture) was filtered through coarse filter paper to remove particles before being blown away with a Rotary evaporator (Heidolph, Germany). The resulting extracts were used for antimicrobial testing. Antimicrobial activity of the strains was evaluated against Pseudomonas aeruginosa ATCC 27853, Enterobacter cloaceae ATCC2468, Enterococcus feacalis ATCC51299, Escherichia coli ATCC2471, Klebsiella pneumoniae ATCC700603, Salmonella typhimurium ATCC13311, Serretia marcescens ATCC13880, Staphylococcus epidermis ATCC14990, Staphylococcus aureus ATCC25923, Bacillus subtilis ATCC 6633, Streptococcus faecalis ATCC 9790, Yersinia pestis ATCC 19428 and Candida albicans ATCC10351. One-way analysis of variance (ANOVA) was run to determine any significant differences in the study groups by Duncan multiple range test was performed through SPSS. Results: Dried hazelnut extract was effective in all of them, while fresh hazelnut extract was only effective on Candida albicans ATCC10351 and Klebsiella pneumoniae ATCC700603 (F (3, 8) = 65.45, p<0.05). The most effective zone of inhibition obtained from dried nut extract was determined on Streptococcus faecalis ATCC 9790 (F (3, 8) = 15.07, p<0.05). It was determined to be above Klebsiella pneumoniae ATCC700603 in the least inhibition zone (F (3, 8) = 46.33, p<0.05). Conclusion: After the necessary scientific research are carried out, hazelnut oil, which is produced under appropriate conditions, can turn into a market with high potential for related companies around the world and for Turkey in terms of cosmetic products and with the development of antibiotics.
Keywords : Antimikrobiyal, Fındık, Mikrobiyoloji, Tombul fındık, Fındık ekstraktı