Effects of Insulin Lispro on Ram Semen Cryopreservation

Authors : Nail Tekin Önder, Taygun Gökdemir, Muhammet Can Kiliç, Savaş Yildiz, Yavuz Öztürkler

Pages : 439-443

Doi:10.30607/kvj.1347383

View : 57 | Download : 101

Publication Date : 2023-09-30

Article Type : Research Paper

Abstract :In the present study, it was aimed to evaluate the effects of semen extender with added insulin on ram semen at the post-thaw stage. Semen was collected from 5 Tuj rams five times with an electro-ejaculator. Experimental groups were designated as control group and groups containing 15 IU and 20 IU insulin lispro added semen extenders. At the post-thaw stage, motility and plasma membrane integrity were evaluated by microscopy. Acrosome integrity (Fitc-Peanut Agglutinin) and mitochondrial membrane potential (Rhodamine 123) were assessed by dual staining with propidium iodide (PI) using flow cytometry. It was found that motility and plasma membrane functional integrity were better preserved in the control group (p 0.05), but acrosome integrity was negatively affected in the 20 IU insulin group (p<0.05). Mitochondrial membrane potential was higher in the group containing 20 IU insulin (p<0.05). As a result, it was thought that energy metabolism was stimulated in ram semen frozen with insulin-added extenders, and the semen was better preserved than the control group.
Keywords : Tuj, Koç sperması, İnsülin, Kriyoprezervasyon