Pit1/Hinfi Polymorphism in Holstein Cattle in Afyonkarahisar Province

Authors : Pelin Gezer Bilecen, Eda Demirtaş, Metin Erdoğan, Cevdet Uğuz

Pages : 386-392

Doi:10.30607/kvj.1485628

View : 44 | Download : 64

Publication Date : 2025-01-03

Article Type : Research Paper

Abstract :Pit1 (Pituitary Specific Transcription Factor-1) gene has been shown to be resided on the centromeric region of chromosome 1 in cattle. This gene, also known as POUF1F has responsible for development of pituitary gland and hormone expression in mammals. Pit1 deficiency causes a dwarfism phenotype in mice, dogs and fish. Previous studies have demonstrated to associated with polymorphisms of the Pit1 gene and growth and reproductive performance and milk, meat yield traits. In the cattle, a HinfI polymorphism on Pit1 gene (c.1178G>A) have been reported that there is an association between milk and reproduction yield parameters. In this study, we aimed existence and distribution of Pit1/HinfI polymorphism in 81 head Holstein cattle in Afyonkarahisar province. In our study, we defined that there were 5 AA, 30 AG and 46 GG genotypes in our survey population. We also detected to frequency of A allele as 0.25 and the G allele as 0.75. We calculated to genetic index values such as PIC (0.3027418) and Heterozygosity (0.3742044) using RStudio package. Chi-square value was found 0.049 and was exhibited to survey population in Hardy-Weinberg equilibrium. The Pit1/HinfI polymorphism is a potential option for use in marker-assisted selection studies in light of these findings for Holstein cattle in Afyonkarahisar province.
Keywords : HinfI, Holstein sığır, PCR-RFLP, Pit1