Assessment of the Impact of Curcumin on Cell Cultures Derived from the Primary Intervertebral Disc Tissue in Humans

Authors : Mehmet ALBAYRAK, İbrahim YILMAZ, Muharrem Furkan YÜZBAŞI, Hande AKALAN, Duygu YAŞAR ŞİRİN, Numan KARAARSLAN, Hanefi ÖZBEK, Ozkan ATES

Pages : 49-55

View : 37 | Download : 53

Publication Date : 2023-08-31

Article Type : Research Paper

Abstract :Aim: Degenerative disc disease in the lumbar spine is widely observed. Degenerative disc diseases are among the causes of low back pain in older age. Modern drug discovery studies have aimed to identify potential molecules that target multiple pathways with a safer profile against degeneration. This study aimed to evaluate the effects of curcumin, a natural phenolic compound, on primary cell cultures prepared using intervertebral disc insert ignore into journalissuearticles values(IVD); tissues resected during the surgeries of patients with lumbar disc herniation. Materials and Methods: Primary cell cultures were prepared using human IVD tissues of eight patients. Untreated groups served as the control and curcumin-treated groups as the study sample. In-vitro cytotoxicity analyses were performed in all groups. Acridine orange insert ignore into journalissuearticles values(AO);/propidium iodide insert ignore into journalissuearticles values(PI); and Janus Green B staining were performed to evaluate cell surface morphologies. One-way analysis of variance and Tukey HSD, a multiple comparison test, were used to assess the obtained data. Results: Proliferation slightly increased as of 24 h in the curcumin-treated samples, but decreased in the 48 and 72 hour curcumin-treated samples compared to the control samples. The obtained results were statistically significant insert ignore into journalissuearticles values(p<0.05);. Additionally no cytotoxicity was observed according to morphological evaluations. Conclusion: This research is an in-vitro experimental study. However, this natural and non-toxic pleiotropic agent can be targeted to cell-damaged sites through appropriate drug delivery systems. It may thus be a safe treatment option for the regeneration of degenerated lumbar IVD cells in the future.
Keywords : Anulus fibrozus, Çoğalma, Kurkumin, Nükleus pulpozus, Primer hücre kültürü, Sitotoksisite