Nephroprotective effect of Resveratrol against Methotrexate -induced renal toxicity in female rats

Authors : Şükriye YEŞİLOT, Meltem ÖZGÖÇMEN

Pages : 123-133

Doi:10.24998/maeusabed.1136994

View : 12 | Download : 8

Publication Date : 2022-08-26

Article Type : Research Paper

Abstract :The study purposed to appraise the nephroprotective effects of resveratrol-insert ignore into journalissuearticles values(RES); in relation to methotrexate-insert ignore into journalissuearticles values(MTX);-induced renal toxicity in female rats. The animals were allocated into three groups with six in each group: control, MTX:insert ignore into journalissuearticles values(15 mg/kg, only a dose, i.p);, MTX+RES group: insert ignore into journalissuearticles values(15 mg/kg MTX, only a dose, i.p + 20 mg/kg RES, only a dose daily, oral gavage, 7 days);. The nephroprotective efficacy was interpreted by measuring biochemical parameters such as serum renal function markers insert ignore into journalissuearticles values(uric acid, BUN and creatinine);, total oxidant insert ignore into journalissuearticles values(TOS); and antioxidant status insert ignore into journalissuearticles values(TAS); in renal homogenates. Moreover, the effect of RES on kidneys was appraised by histopathological and immunohistochemical analyzes. In MTX-induced rats, RES treatment exhibited its nephroprotective effects with a significant increase in renal TAS as well as a significant decrease in serum BUN and renal TOS levels. In parallel with the biochemical data, it was observed that RES had a protective effect in the histological staining findings. Immunohistochemically, it was determined that TNF-α, one of the indicators of systemic inflammatory response, decreased with RES-treatment. The findings of the study show that RES administration 1 hour before MTX injection to rats has a curative effect on renal damage.
Keywords : Metotreksat, Resveratrol, Böbrek toksisitesi, dişi sıçanlar