Phenotypic and genotypic characterisation of pepper genotypes for Tomato Spotted Wilt Orthotospovirus reaction and resistance

Authors : Ömer Faruk ÖZDEMİR, Eminur ELÇİ

Pages : 59-67

Doi:10.29136/mediterranean.1034291

View : 23 | Download : 7

Publication Date : 2022-08-02

Article Type : Research Paper

Abstract :In order to determine the effects of Tomato spotted wilt orthotospovirus insert ignore into journalissuearticles values(TSWV); on the yield and fruit quality parameters of some common local and commercial pepper insert ignore into journalissuearticles values(Capsicum annuum L.); genotypes under greenhouse conditions, mechanical inoculations were performed, and virus infections were tested by DAS-ELISA and RT-PCR after the inoculations. Based on the DAS-ELISA results, 95% of the inoculated plants were found to be positive for TSWV, in accordance with the expected amplicon size insert ignore into journalissuearticles values(276 bp); obtained by RT-PCR results. Infection of TSWV caused significant insert ignore into journalissuearticles values(p≤0.05); reduction in fruit number insert ignore into journalissuearticles values(45.97-100%);, fruit weight insert ignore into journalissuearticles values(66.38-98.77%);, fruit width insert ignore into journalissuearticles values(63.77-96.52%);, fruit length insert ignore into journalissuearticles values(31.97-93.514%);, flesh thickness insert ignore into journalissuearticles values(28.64-82.41%);, fruit firmness insert ignore into journalissuearticles values(5.82-94.43%);, fruit colour insert ignore into journalissuearticles values(1.62-7.79%);, and total yield insert ignore into journalissuearticles values(68.62-100%); in infected plants. The best performance against TSWV was observed on cv. Yalova Çarliston 341, while the lowest was observed on cv. Bora 77 among the tested pepper cultivars Yalova Çarliston 341, Yalova Tatlı Kıl, Yalova Yağlık 28, Mazamort, Sera Demre 8, Üçburun, Geyikboynuzu, Bor Biberi, Bora 77 and 153-227. Moreover, the incidence of Tsw resistance gene was investigated by molecular analysis using CAPS marker SCAC568 with TaqI restriction enzyme digestion; however, Tsw gene could not be detected in any of the tested cultivars except wild type C. chinense and resistant genotype cv.153-227. This study reveals the effects of TSWV in common pepper genotypes and will be important for virus resistant breeding studies.
Keywords : Pepper, TSWV, RT PCR, Tsw resistance gene, CAPS